AA100 AutoAnalyzer

Our lowest footprint segmented flow analyzer, offering inline sample preparation and automated, low-level colorimetric analysis for up to two methods.

Compact, Automated Two-Channel Analysis

The compact dual channel AA100 auto analyzer is the ideal chemistry analyzer for laboratories running only 1 or 2 dedicated chemistries. Like the bigger AA500 AutoAnalyzer it is designed specifically for environmental and industrial testing laboratories. Using Segmented Flow Analysis techniques (a version of Continuous Flow Analysis) the AA100 AutoAnalyzer fully automates repetitive and complex sample analysis steps, including sample distillation. With its very small footprint, this compact auto analyzer will fit into any bench top, in any orientation. Despite its compact size and simplicity of use, the AA100 Auto Analyzer delivers you some of the same features as the bigger AA500 and QuAAtro39 AutoAnalyzers including the low detection limits, exceptional reproducibility, glass coils, digital photometer, LED light source, and a very low cost per test.

AA100 AutoAnalyzer Brochure

View the full specifications

Why select the AA100?

Automation

Automated colorimetric analysis of a wide range of nutrients and analytes
Ammonia analysis by in-line gas diffusion eliminates need for distillation as well as pH and salinity issues.
Integrated distillation manifolds for cyanide, phenol, and ammonia
ASX-series samplers designed by SEAL Analytical for improved segmented flow analysis sampling

Compliance

Segmented reaction stream allows for a complete reaction between reagents and sample for maximum sensitivity
LIMS-compatible software available in standard and GLP versions
24-bit high resolution detectors provide ultra-low detection limits and wide dynamic ranges
High-precision detection with bubble-through technology and LED light sources

Efficiency

Ability to add samples during the run
Small footprint ideal for limited bench space
Easy operation and intuitive software
Low-cost analysis

Support

Visual manuals and descriptive checklists
Immediate support available from SEAL chemists via email, phone, screenshare, or video call
In-depth training during installation
Guides and webinars available for continued learning

Highlights of the AA100

Small Footprint & Compact Design

With the smallest footprint in SEAL’s analyzer suite at less than 2 square feet, the AA100 accommodates small labs and low bench space while providing powerful automation options.

Inline Sample Preparation

In addition to analysis, the AA100 can perform inline distillation, dialysis, and dilution. Eliminate distillation steps entirely for ammonia with inline gas diffusion options for further streamlined analysis.

Low Reagent Usage

Utilizing segmented flow analysis, SEAL flow analyzers use less reagent than other variations of continuous flow analysis. Segmented flow also brings the reaction to reach complete color formation, allowing the signal to reach a steady state for precise measurement.

High Performance Digital Photometer

The AA100 digital photometer utilizes low-maintenance LED light sources and provides high sensitivity, wide dynamic ranges, and low detection limits.

How the AA100 Works

Startup and Baselines

Samples are loaded and scheduled

Sampling begins

Reaction underway

Detection takes place

Post-run dilutions and shutdown

Step 1

Startup and Baselines

The system is powered on and wash solution is pumped through the manifold. After the baseline has stabilized, reagents are introduced through the peristaltic pump and into the manifold via flow-rated pump tubes. A stable baseline is achieved before analysis begins.

Step 2

Samples are loaded and scheduled

A sample list or sequence is entered into the software, either imported directly from LIMS or a spreadsheet, or entered manually. If using a syringe diluter, auto-dilution factors are assigned. Samples are loaded onto the sampler.

Step 3

Sampling begins

Once the run is started, the standards and samples are pulled sequentially through the sample probe by the peristaltic pump and flow-rated pump tubes. The sampler returns to the wash bath between samples for cleaning. The sample can be split into two channels as it approaches the pump for simultaneous analysis of multiple analytes. As the sample enters the manifold, an air bubble is injected every 2 seconds to provide additional separation between samples.

Step 4

Reaction underway

As the samples pass through the manifold, precise volumes of reagents are injected at specific points, pulled through the peristaltic pump by their own flow-rated pump tubes. The reaction solution passes through a range of apparatuses on the manifold such as mixing coils, dialyzers, gas diffusion blocks, and more to facilitate the colorimetric reaction.

Step 5

Detection takes place

The reaction solution reaches peak color development before passing into the flowcell. Using a high-resolution LED digital photometer, constant absorbance readings of the flow are measured and plotted on a chart in the AACE software in real time. As the colored reaction solution enters and leaves the detector, peaks are formed on the chart. Peak height is then measured and compared to the calibration to calculate a concentration value of the sample.

Step 6

Post-run dilutions and shutdown

After the run completes, over-range samples can be automatically diluted using a syringe diluter and reanalyzed if desired. At the end of the run, the pump can be programmed to a slow mode to reduce reagent use after the run is complete.

Powerful AACE Software

All instrument control and data acquisition are managed through powerful and intuitive SEAL AutoAnalyzer Control and Evaluation (AACE) software. Designed in-house at SEAL Analytical, AACE is compatible with Windows 7 - 11 and offers regular updates keeping up with new operating systems. Offering LIMS import and export compatibility and GLP-compliance, AACE is compatible with all laboratories' data-handling needs.

AA100 AutoAnalyzer Methods

Explore a wide range of our most popular methods for environmental, agricultural, industrial, and other applications on the AA100 AutoAnalyzer. Don’t see what you’re looking for? Contact our team to discuss our full method options.

SEAL Analytical has more than 1,000 documented segmented flow analyzer methods, with new applications regularly being developed in our laboratories. USEPA, ASTM, ISO, AOAC, DIN, CORESTA and other international regulatory compliant methods available.

EPA Methods

Analyte	SEAL Method #	Lowest Range	Highest Range
Ammonia	E1001	0 - 600 μg/L as N	0 - 14 mg/L as N
Cyanide	E022	0 - 50 µg/L as CN	0 - 500 µg/L as CN
Nitrate	E1003	0 - 350 μg/L as N	0 - 8 mg/L as N
Nitrite	E1002	0 - 250 µg/L as N	0 - 2.5 mg/L as N
Nitrogen, total Kjeldahl	E1006	0 - 0.75 mg/L as N	0 - 12 mg/L as N
Nitrogen, total Kjeldahl	E1006	0 - 0.75 mg/L as N	0 - 12 mg/L as N
Phosphate	E1004	0 - 750 μg/L as P	0 - 10 mg/L as P
Phosphorus, total Kjeldahl	E1005	0 - 1.0 mg/L as P	0 - 9.0 mg/L as P

Methods with Inline Gas Diffusion (eliminating need for manual distillation)

Analyte	SEAL Method #	Lowest Range	Highest Range
Ammonia (Phenate Reagent)	E020	0 - 0.8 mg/L as N	0 - 20 mg/L as N
Ammonia (Salicylate Reagent)	E021	0 – 1.2 mg/L as N	0 – 14 mg/L as N
Nitrogen, total Kjeldahl	E1009	0.078 - 10 mg/L as N	0.078 - 10 mg/L as N

ISO Methods

Analyte	SEAL Method #	Lowest Range	Highest Range	Inline Pretreatment
Ammonia	E001	0 - 800 μg/L as N	0 - 15 mg/L as N
Ammonia	E007	0 - 2 mg/L as N	0 - 13 mg/L as N
Ammonia	E035	0 - 1000 µg/L as N	0 - 30 mg/L as N	Dialysis
Cyanide, total	E017	10 - 100 µg/L as CN	100 - 1000 µg/L as CN	UV Digestion, Distillation
Cyanide, total	E023	0 - 64 µg/L as CN	0 - 1500 µg/L as CN	UV Digestion, Gas Diffusion
Cyanide, WAD	E018	0 - 100 µg/L as CN	0 - 1000 µg/L as CN	Distillation
Cyanide, WAD	E023	0 - 64 µg/L as CN	0 - 1500 µg/L as CN	UV Digestion, Gas Diffusion
Nitrate	E003	0 - 200 μg/L as N	0 - 5 mg/L as N
Nitrate	E008	0 - 6 mg/L as N	0 - 6 mg/L as N
Nitrite	E002	0 - 150 μg/L as N	0 - 4 mg/L as N
Nitrite	E048	0 - 200 µg/L as N	0 - 3.2 mg/L as N	Dialysis
Nitrogen, total	E005	0 - 200 µg/L as N	0 - 6 mg/L as N
Phenol	E009	0 - 200 μg/L as Phenol	0 - 5 mg/L as Phenol	Distillation
Phosphate	E004	0 - 800 μg/L as P	0 - 12 mg/L as P
Phosphate	E036	0 - 1000 µg/L as P	0 - 40 mg/L as P	Dialysis
Phosphorus, total	E006	0 - 800 μg/L as P	0 - 12 mg/L as P

Other models

AutoAnalyzer II (Legacy System)

Introduced in 1969, the Technicon AutoAnalyzer II was the 20th century's best known and most successful Continuous Flow Automated Analyzer, and the instrument that most EPA methods reference. Predecessor to the SEAL Analytical AA3 and AA500, it laid the groundwork for the widespread popularity of segmented flow analysis.

Learn more

AA3 HR AutoAnalyzer (Legacy System)

Succeeded by the AA500, the AA3 AutoAnalyzer brought segmented flow analysis into the Digital Age. Following the success of the AutoAnalyzer II from Technicon and Bran+Luebbe, the AA3 and AA3 HR became the standard automated analyzer for nutrient analysis in seawater and tobacco analysis, and a key system for water, wastewater, drinking water, fertilizer, soil, and plant analysis.

Learn more

QuAAtro39 AutoAnalyzer

Incredibly powerful and compact, the QuAAtro39 AutoAnalyzer offers micro or macro flow hydraulics, heated manifolds, high resolution detectors and advanced software. This high performance microflow chemistry analyzer is ideal for the detection of ultra-low nutrients in water, wastewater, seawater, soil, plant extracts, tobacco and wine.

Learn more

AA500 AutoAnalyzer

The AA500 is the top-of-the-line AutoAnalyzer incorporating the latest innovations to deliver your laboratory the freedom of total automation while achieving very high precision and the lowest detections levels. The AA500 is the AutoAnalyzer you’ve been waiting for- total automation, ultralow detection, multitest chemistries, high throughput.

Learn more

Frequently Asked Questions

Below, we’ve listed a few frequently asked questions that will help you better understand our Continuous Flow Analyzers. If you don’t find the answer you're looking for, please don't hesitate to contact our support team for further assistance.

What is the throughput of SEAL Analytical’s continuous flow analyzers?

Our continuous flow analyzers can analyze samples at up to 120 samples/hour per channel, for a total of up to 600 tests per hour.

What is the advantage of injecting air bubbles in segmented flow analysis?

Our continuous flow analyzers utilize segmented flow analysis, injecting controlled bubbles at a regular interval which provides several benefits. The bubbles reduce carryover by adding physical separation between sample and wash solution, they improve the mixing of sample and reagent in-between air bubbles, they scrub the inner surface of the glass coils as the move along, and they provide a visual of the flow through the system for easy fluidic checks.

How does segmented flow analysis compare to flow injection analysis?

Segmented flow analysis and flow injection analysis are both types of continuous flow analysis. In segmented flow analysis, a gas bubble is injected into the sample stream at regular intervals (see question above). In flow injection analysis, samples an reagents are pumped into a manifold under pressure without any air bubble injection. Each type of continuous flow analysis uses different methods for analyzing the peaks charted as samples pass through the detector. We discuss these differences in detail here.

What maintenance is involved on a segmented flow analyzer?

With minimal moving parts, segmented flow analysis has an advantage in requiring little routine maintenance. The maintenance involved includes rinsing out the channels after each run using the defined cleaning protocols for the chemistry ran on the instrument, releasing the platen so the pump tubes are not compressed when not in use, changing the flow-rated pump tubes at 200 hours of use, and keeping the pump clean and lubricated. Each SEAL analyzer comes with checklists and visual guides for these easy procedures, and our technical support team is always happy to assist with any questions.